Home Biology Biotechnology Principles and Processes NCERT Examplar The role of DNA ligase in the construction o…
Biology Biotechnology Principles and Processes NCERT Examplar Single Correct MCQ
Published on: August 14, 2026

The role of DNA ligase in the construction of a recombinant DNA molecule is:

A
Formation of phosphodiester bond between two DNA fragments.
B
Formation of hydrogen bonds between sticky ends of DNA fragments.
C
Ligation of all purine and pyrimidine bases.
D
None of the above

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Text Solution

Verified by Experts
The correct answer is:
A

DNA ligase (joining or sealing enzymes) are also called genetic gum. They join two individual fragments of double-stranded DNA by forming phosphodiester bonds between them. Thus they help in sealing gaps in DNA fragments. Therefore, they act as a molecular glue.

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