A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
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The process of making recombinant DNA molecule involves the introduction of a desired gene into the DNA of a host that will produce the desired protein.
Inducing a cloned eukaryotic gene to function in a prokaryotic host can be difficult sometime. The presence of long non-coding introns in eukaryotic genes may prevent correct expression of these genes in prokaryotes, which lack RNA-splicing machinery.
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