Insertion of a foreign DNA at Brahmi site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards:
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Tetracycline: The BamHI site is located specifically within the tetracycline resistance gene (tet 
) in pBR322.
• Insertional Inactivation: Inserting foreign DNA here disrupts the gene, causing the cell to lose its resistance to tetracycline.
• Ampicillin: The 
gene remains functional because its restriction sites are different (Pstl and Pvul).
Gentamycin: pBR322 does not carry a resistance gene for this antibiotic.
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