Read the following and choose the option with all the correct statements.

(i) Biotechnology deals with techniques of using live organisms or enzymes from organisms to produce products and processes useful to humans.

(ii) EFB has given a definition of biotechnology that encompasses both traditional view and modern molecular biotechnology.

(iii) The techniques of genetic engineering include creation of recombinant DNA molecule, by use of gene cloning and gene transfer.

(iv) Making multiple identical copies of any template DNA is called cloning.

(v) The autonomously replicating circular extra-chromosomal DNA in bacteria is probe.

(vi) The first recombinant DNA was made by linking gene encoding antibiotic resistance with native plasmid of Salmonella typhimurium.

(vii) Stanley Cohen and Herbert Boyer made first recombinant DNA molecule in 1927.

(viii) Molecular scissors in rDNA technology are the enzyme restriction enzymes.

(ix) DNA ligase enzyme acts on cut DNA molecules and join their ends.

(x) The two enzymes responsible for restricting the growth of bacteriophage in E.coli are restriction endonuclease and methylase enzyme.

(xi) The first restriction endonuclease was Bam HI.