Mark the given statements True (T) or False (F).

(i) Origin of replication (ori) is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cell.

(ii) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.

(iii) When pBR322 vector is used for transforming recombinant DNA molecule using BamHI as restriction enzyme, then recombinant cell will grow on tetracycline antibiotic containing medium, but not on medium containing ampicillin.

(iv) When a recombinant DNA is inserted within the coding sequence of an enzyme β-gatactosidase, it results into inactivation of enzyme, which is referred to as insetional inactivation.

(v) In insertional inactivation of coding sequence of β-galactosidase, the presence of chromogenic substrate gives blue coloured colonies if the plasmid in bacteria does not have an insert.

(vi) Agrobacterium tumifaciens, a pathogen of several monocot plants is able to deliver a piece of DNA called T-DNA to transform normal plant cells into a tumor.

(vii) Since DNA is hycrophobic molecule, it cannot pass through cell membranes.

(viii) Bacterial cells can be made to take recombinant DNA by incubating it with DNA on ice, followed by heat shock at 42 o C and then again putting them on ice.

(ix) In micro-injection method, recombinant DNA is directly injected into the nucleus of plant cell.

(x) In biolistics method, the cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.

(xi) Lysozyme can be used to break bacterial cell; cellulose to break plant cell and chitinase to break fungal cell.

Choose the correct option given below: