The role of DNA ligase in the construction of a recombinant…

Biology Biotechnology Principles and Processes Statement Based Questions Single Correct MCQ

Published by: CGP EDU Academic Team

Published on: August 14, 2026

The role of DNA ligase in the construction of a recombinant DNA molecule is

(I) formation of phosphodiester bond between two DNA fragments.

(II) formation of hydrogen bonds between sticky ends of DNA fragments.

(III) ligation of all purine and pyrimidine bases.

I and II

II and III

I and III

I only

Text Solution

Verified by Experts

The correct answer is:

DNA ligase’s main role is to join DNA fragments by forming phosphodiester bonds between the sugar-phosphate backbones (Statement I).

(II) is incorrect → hydrogen bonds between sticky ends form on their own; ligase does not form them.

(III) is incorrect → ligase does not join individual bases.

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