Arrange the following steps of the recombinant DNA procedure, including blue-white screening, in the correct order:
(E) 
(E) 
(E)
(E) Identification of blue and white colonies based on the presence of the DNA insert
Options:
(E) 
Text Solution
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The correct order of steps in the recombinant DNA procedure, leading to the successful identification of transformants containing the desired DNA insert is
• Cutting: The plasmid vector and donor DNA are cut with restriction enzymes to create compatible ends.
• Ligation: The donor DNA fragments are ligated into the plasmid vector.
• Transformation: The recombinant plasmid is introduced into competent 
. coli cells.
• Screening: Transformants are screened on plates with a chromogenic substrate (e.g., X-Gal).
• Identification: Blue and white colonies are identified based on the presence of the DNA insert.
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