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Download Comprehensive Syllabus

NSEB

NSEB Overview

The National Standard Examination in Biology (NSEB) is India’s most prominent school-level biology competition, held annually to identify students with a deep interest and exceptional talent in biological sciences. It serves as the first stage of India’s Biology Olympiad program and is a gateway to representing the country at the International Biology Olympiad (IBO).

The exam is organized by the Indian Association of Physics Teachers (IAPT), in collaboration with the Homi Bhabha Centre for Science Education (HBCSE), which manages the subsequent stages of the selection process.

Exam Details
EXAM NSEB
Full Form National Standard Examination in Biology
Day & Date Sunday
November 23, 2025
Time of Exam 2.30 pm to 4.30 pm
Venue Respective NSE centre

NSEB Exam Pattern & Eligibility

Details Information
Eligibility (i.e) who can appear for 1. Must be eligible to hold an Indian passport. 2. Date of birth between 1 July 2006 and 30 June 2011, both days inclusive. 3. Must be residing and studying in India since 30 November 2023 or earlier. OR Must be studying in an Indian school system since 30 November 2023 or earlier. 4. Must not have completed (or scheduled to complete) class 12 board examination earlier than 30 November 2025. 5. Must not have commenced (or planning to commence) studies in a university or equivalent institution by 1 June, 2025. 6. Must not be appearing in NSEJS 2025.
Level of Question Paper Is broadly equivalent to senior secondary level (up to and including Class XII) of CBSE India.
Language of Question Paper Question paper will be in English, Hindi, Gujarati, Bangla, Kannada, Tamil & Telugu or any Indian Language provided 300 students OPT for it (option during registration)
Question Paper Pattern A 48 multiple choice questions with one alternative correct. +3 marks credit for correct choice. -I mark penalty for incorrect choice.
Question Paper Pattern B 12 multiple choice questions each of six marks with one or more than one correct alternatives. To get credit, all the correct option(s) and no incorrect option(s) should be marked.

Why is NSEB Important?

  • It is the gateway to the International Biology Olympiad (IBO).
  • Tests conceptual understanding, analytical skills, and application-based knowledge in biology.
  • Around Top 300–500 students qualify for INBO (Indian National Biology Olympiad).
  • Enhances readiness for NEET, KVPY, and IISER/NISER entrance tests.
  • Promotes in-depth learning of genetics, ecology, physiology, and evolution beyond school level.
  • Opportunity to attend OCSC and pre-international training camps.
  • Recognized by top science institutes and scholarship programs.
  • Encourages future careers in biotechnology, medicine, life sciences, and research.
  • Builds strong research orientation and interest in experimental biology.

NSEB Syllabus

In addition to following, general understanding of Science topics studied till Class 10 and Mathematics topics studied till Class 12 is expected

Section Topics
I. Diversity of Living Organisms
  • The Living World: What is living? Biodiversity; Need for classification; Three domains of life; Taxonomy and systematics; Concept of species and taxonomical hierarchy; Binomial nomenclature and tools for study of taxonomy.
  • Biological Classification: Five kingdom classification; Salient features and classification of Monera, Protista and Fungi into major groups; Lichens, Viruses and Viroids.
  • Plant Kingdom: Salient features and classification of plants into major groups - Algae, Bryophyta, Pteridophyta, Gymnospermae and Angiospermae.
  • Animal Kingdom: Salient features and classification of animals, non-chordates up to phyla level and chordates up to class level.
II. Structural Organization in Animals and Plants
  • Morphology and Anatomy of Flowering Plants: Morphology and modifications; Morphology of different parts of flowering plants: root, stem, leaf, inflorescence, flower, fruit and seed; Anatomy and functions of different tissues and tissue systems.
  • Structural Organisation in Animals: Animal tissues; Morphology, anatomy and functions of different systems (digestive, circulatory, respiratory, nervous and reproductive) of animals.
III. Cell: Structure and Function
  • Cell - The Unit of Life: Cell theory and cell as the basic unit of life; Structure of prokaryotic and eukaryotic cells; Plant cell and animal cell; Cell envelope; Cell membrane, cell wall; Structure and function of all cell organelles.
  • Cellular Respiration: Glycolysis, fermentation (anaerobic), TCA cycle and electron transport system (aerobic); Energy relations – number of ATP molecules generated; Amphibolic pathways; Respiratory quotient.
  • Biomolecules: Chemical constituents of living cells – biomolecules, structure and function of proteins, carbohydrates, lipids, nucleic acids; Enzymes – types, properties, enzyme action.
  • Cell Cycle and Cell Division: Cell cycle, mitosis, meiosis and their significance.
IV. Plant Physiology
  • Transport in Plants: Movement of water, gases and nutrients; Cell to cell transport – diffusion, facilitated diffusion, active transport; Plant-water relations, imbibition, water potential, osmosis, plasmolysis; Long distance transport of water – absorption, apoplast, symplast, transpiration pull, root pressure and guttation; Transpiration, opening and closing of stomata; Uptake and translocation of mineral nutrients; Transport of food – phloem transport, mass flow hypothesis.
  • Mineral Nutrition: Essential minerals, macro- and micronutrients and their role; Deficiency symptoms; Mineral toxicity; Elementary idea of hydroponics as a method to study mineral nutrition; Nitrogen metabolism, nitrogen cycle, biological nitrogen fixation.
  • Photosynthesis and Respiration in Higher Plants: Photosynthesis as a means of autotrophic nutrition; Site of photosynthesis, pigments involved in photosynthesis (elementary idea); Photochemical and biosynthetic phases of photosynthesis; Cyclic and non-cyclic photophosphorylation; Chemiosmotic hypothesis; Photorespiration; C3 and C4 pathways; Factors affecting photosynthesis; Exchange of gases.
  • Plant - Growth and Development: Seed germination; Phases of plant growth and plant growth rate; Conditions of growth; Differentiation, dedifferentiation and redifferentiation; Sequence of developmental processes in a plant cell; Growth regulators; Seed dormancy; Vernalisation; Photoperiodism.
V. Human Physiology
  • Digestion and Absorption: Alimentary canal and digestive glands; Role of digestive enzymes and gastrointestinal hormones; Peristalsis, digestion, absorption and assimilation of proteins, carbohydrates and fats; Calorific values of proteins, carbohydrates and fats; Egestion; Nutritional and digestive disorders.
  • Breathing and Exchange of Gases: Respiratory system in humans; Mechanism of breathing and its regulation in humans; Exchange of gases, transport of gases and regulation of respiration; Respiratory volume; Disorders related to respiration.
  • Body Fluids and Circulation: Composition of blood, blood groups, coagulation of blood; Composition of lymph and its function; Human circulatory system - structure of human heart and blood vessels; Cardiac cycle, cardiac output, ECG; Double circulation; Regulation of cardiac activity; Disorders of circulatory system.
  • Excretory Products and Their Elimination: Modes of excretion - ammonotelism, ureotelism, uricotelism; Human excretory system - structure and function; Urine formation, osmoregulation; Regulation of kidney function - renin-angiotensin, atrial natriuretic factor, ADH and diabetes insipidus; Role of other organs in excretion; Disorders of excretory system; Dialysis and artificial kidney, kidney transplant.
  • Locomotion and Movement: Types of movement - ciliary, flagellar, muscular; Skeletal muscle - contractile proteins and muscle contraction; Skeletal system and its functions; Joints; Disorders of muscular and skeletal system.
  • Neural Control and Coordination: Neuron and nerves; Nervous system in humans - central nervous system; Peripheral nervous system and visceral nervous system; Generation and conduction of nerve impulse; Reflex action; Sensory perception; Structure and functions of sense organs.
  • Chemical Coordination and Integration: Endocrine glands and hormones; Human endocrine system; Mechanism of hormone action; Role of hormones as messengers and regulators; Hypo- and hyperactivity and related disorders.
VI. Reproduction
  • Reproduction in Organisms: Reproduction, a characteristic feature of all organisms for continuation of species; Modes of reproduction - asexual and sexual reproduction; Asexual reproduction - binary fission, sporulation, budding, gemmule formation, fragmentation; Vegetative propagation in plants.
  • Sexual Reproduction in Flowering Plants: Flower structure; Development of male and female gametophytes; Pollination - types, agencies and examples; Outbreeding devices; Pollen-pistil interaction; Double fertilization; Post-fertilization events - development of endosperm and embryo, development of seed and formation of fruit; Special modes - apomixis, parthenocarpy, polyembryony; Significance of seed dispersal and fruit formation.
  • Human Reproduction: Male and female reproductive systems; Microscopic anatomy of testis and ovary; Gametogenesis - spermatogenesis and oogenesis; Menstrual cycle; Fertilization, embryo development up to blastocyst formation, implantation; Pregnancy and placenta formation; Parturition; Lactation.
  • Reproductive Health: Need for reproductive health and prevention of Sexually Transmitted Diseases (STDs); Birth control - need and methods, contraception and medical termination of pregnancy (MTP); Amniocentesis; Infertility and assisted reproductive technologies - IVF, ZIFT, GIFT.
VII. Genetics and Evolution
  • Heredity and Variation: Mendelian inheritance; Deviations from Mendelism – incomplete dominance, co-dominance, multiple alleles and inheritance of blood groups, pleiotropy; Elementary idea of polygenic inheritance; Chromosome theory of inheritance; Chromosomes and genes; Sex determination - in humans, birds and honey bee; Linkage and crossing over; Sex-linked inheritance; Mendelian disorders in humans.
  • Molecular Basis of Inheritance: DNA as genetic material; Structure of DNA and RNA; DNA packaging; DNA replication; Central dogma; Transcription, genetic code, translation; Gene expression and regulation - lac operon; Genome; DNA fingerprinting.
  • Evolution: Origin of life; Biological evolution and evidences for biological evolution (paleontology, comparative anatomy, embryology and molecular evidences); Darwin's contribution, modern synthetic theory of evolution; Mechanism of evolution - variation (mutation and recombination) and natural selection with examples, types of natural selection; Gene flow and genetic drift; Hardy-Weinberg's principle; Adaptive radiation; Human evolution.
VIII. Biology and Human Welfare
  • Human Health and Diseases: Pathogens; Parasites causing human diseases (malaria, dengue, chikungunya, filariasis, ascariasis, typhoid, pneumonia, common cold, amoebiasis, ringworm) and their control; Basic concepts of immunology - vaccines; Cancer, HIV and AIDS; Adolescence - drug and alcohol abuse.
  • Strategies for Enhancement in Food Production: Improvement in food production: Plant breeding, tissue culture, single cell protein, biofortification, apiculture and animal husbandry.
  • Microbes in Human Welfare: In household food processing, industrial production, sewage treatment, energy generation and microbes as bio-control agents and bio-fertilizers; Antibiotics; Production and judicious use.
  • Biotechnology – Principles, Processes and Applications: Genetic Engineering (Recombinant DNA Technology); Application of biotechnology in health and agriculture: Human insulin and vaccine production, stem cell technology, gene therapy; Genetically modified organisms - Bt crops; Transgenic animals; Biosafety issues, bio-piracy and patents.
IX. Ecology and Environment
  • Organisms and Populations: Organisms and environment: Habitat and niche, population and ecological adaptations; Population interactions - mutualism, competition, predation, parasitism; Population attributes - growth, birth rate and death rate, age distribution.
  • Ecosystems: Patterns, components; Productivity and decomposition; Energy flow; Pyramids of number, biomass, energy; Nutrient cycles; Ecological succession; Carbon fixation, pollination, seed dispersal.
  • Biodiversity and its Conservation: Biodiversity - concept, patterns, importance; Loss of biodiversity; Biodiversity conservation; Hotspots, endangered organisms and extinction.
  • Environmental Issues: Air pollution and its control; Water pollution and its control; Agrochemicals and their effects; Solid waste management; Radioactive waste management; Greenhouse effect and climate change - impact and mitigation; Ozone layer depletion; Deforestation.

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