Which of the following components/processes of recombinant DNA technology are incorrectly paired with its characteristic?
(I) DNA ligase : Multiplication of rDNA molecules.
(II) DNA precipitation out of a mixture of biomolecules : Methanol at room temperature.
(III) Roman number in EcoRI : Indicate the order in which the enzyme was isolated from that strain of bacteria.
(IV) Cohesive ends: Single stranded portions at the ends of DNA fragments obtained after restriction enzyme digestion.
Text Solution
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• DNA ligase joins DNA fragments (forms phosphodiester bonds); multiplication of rDNA occurs via PCR, not by DNA ligase.
• Standard precipitation uses chilled ethanol or isopropanol with salt, not methanol at room temperature.
• Roman number in EcoRI denotes the order of discovery of the enzyme from that strain.
• Cohesive ends are single-stranded overhangs at DNA fragment ends.
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